TLS Online TPP Program

#Question id: 3817


DNA replication is studied in a newly discovered bacterium. It takes 30 min for the bacterium to complete a round of replication at 37oC. A Meselson-Stahl-type experiment was performed. The bacteria were grown for several generations in 14N-medium and then switched to 15N-medium. The DNA molecules were then analyzed.

If the mechanism of DNA replication in this bacterium were dispersive, what results would be found when the double-stranded DNA was analyzed in a CsCl gradient after two generations in 15N-medium?

#Unit 3. Fundamental Processes
  1. One band would be observed containing all the DNA.

  2. Two bands would be observed containing equal amounts of DNA.

  3. Two bands would be observed containing unequal amounts of DNA.

  4. The DNA cannot form any bands if replication is dispersive.