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TLS Online TPP Program

#Question id: 3821

Which mechanism contributes to accuracy during DNA replication?

#Unit 3. Fundamental Processes

  1. The mismatch repair system recognizes an incorrect base-pair and corrects the mistake in the non-methylated strand.

  2. Using primers increases accuracy because the first nucleotides in a new nucleic acid chain are more likely to be correct.

  3. All DNA polymerases have a 5′→ 3′ exonuclease activity which can remove incorrect nucleotides during replication.

  4. Base-stacking between nucleotides and the template DNA controls insertion of the correct nucleotide.

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TLS Online TPP Program

#Question id: 4064

#Unit 3. Fundamental Processes

Which of the following mutations can result in a reduction of β-galactosidase?
TLS Online TPP Program

#Question id: 4065

#Unit 3. Fundamental Processes

The NtrC protein from E. coli

a. is a σ54 RNA polymerase.  b. stimulates transcription of the glnA gene.

c. is activated by a protein kinase called NtrB.

d. binds both to an enhancer site upstream of the glnA gene and to RNA polymerase.

e. has ATPase activity.
TLS Online TPP Program

#Question id: 4066

#Unit 3. Fundamental Processes

Specific DNA control elements in promoters can

a. interact with general transcription factors.

b. interact with repressor proteins. 

c. interact with activator proteins.

d. remain unavailable because of condensed chromatin.
TLS Online TPP Program

#Question id: 4067

#Unit 3. Fundamental Processes

Reporter genes are used to

a. express enzymes that are not easily assayed in cell extracts.

b. express enzymes that are easily assayed in cell extracts.

c. characterize DNA control elements. 

d. characterize reporter plasmids.
TLS Online TPP Program

#Question id: 4068

#Unit 3. Fundamental Processes

The three eukaryotic RNA polymerases can be distinguished by

a. the types of genes they transcribe.

b. the number and types of large subunits.

c. their differential sensitivities to cycloheximide.

d. their differential sensitivities to α-amanitin.
TLS Online TPP Program

#Question id: 4069

#Unit 3. Fundamental Processes

Which of the following can be identified using a series of promoter linker scanning mutations?

a. areas of the promoter that are non-essential

b. areas of the promoter that are essential

c. the presence of separate transcriptional control regions

d. spacing constraints on separate transcriptional control regions