State whether the following statements is true/false regarding DNA replication.

1. Proteins bind to both origin sites and termination sites on DNA during DNA replication.

2. In the Sanger method for DNA sequencing, the gels are read from bottom to top, since the smallest fragments travel farthest in the sequencing gel.

3. DNA polymerase I which is inhibited by dideoxynucleoside triphosphates is used in the Sanger method for DNA sequencing.

4. Sanger DNA sequencing requires inhibition of DNA polymerization by a specific dideoxynucleoside every time it is copied in the replication process.

5. The Klenow fragment can be used for DNA sequencing if proteins such as SSB protein are added.

#Question id: 4404

#Unit 3. Fundamental Processes

The yeast two-hybrid system can be used to identify

a. proteins that interact with a known or “bait” protein.

b. proteins that interact with a “fish” domain.

c. cDNAs that encode interacting proteins.

d. co-activators and co-repressors.

#Question id: 4405

#Unit 3. Fundamental Processes

Which of the following can occur when steroid hormone binds to its cognate receptor?

a. conformational changes in the receptor

b. translocation of the receptor out of the cell membrane

c. binding of the receptor to DNA response elements

d. stimulation of transcription

#Question id: 4406

#Unit 3. Fundamental Processes

An electrophoretic mobility shift assay (EMSA) was conducted to check for the binding of TFIIB to a segment of a DNA promoter region. Which of the following approaches could be used to confirm that TFIIB is indeed binding?

#Question id: 4407

#Unit 3. Fundamental Processes

The general transcriptional factors are used by all sets of RNA Polymerase. Which of the following GTF is/are commonly used by all RNA Polymerases?

A. TBP

B. TFIID

C. TFIIIC

D.TFIIE

E. TFIIH

#Question id: 4408

#Unit 3. Fundamental Processes

an example/s of in vivo “molecular mimicry” is/are

I. EF-G–GDP and EF-Tu–GTP–tRNA

II. TAF42 -TAF62 and H3.H4 tetramer

III. Tyrosinyl-tRNA and Puromycin

#Question id: 4409

#Unit 3. Fundamental Processes

The torpedo model of termination is

A. Degradation of the second RNA as it emerges from the RNA polymerase

B. It Involves Rat1/Xrn2 to degrade and dislodge the RNA Polymerase

C. Inhibition of second replication cycle as Mitotic cdkc is freed

D. It Involves APC/C cdh1 to Mitotic cdkc