State whether the following statements is true/false regarding DNA replication.

1. A short primer is added to the DNA polymerase reaction mix for Sanger DNA sequencing that has a 5ʹ free hydroxyl to which new nucleotides can be added.

2. In a sequencing gel, all fragment in one of the four lanes terminates in the same type of nucleotide base.

3. Eukaryotic cells contain at least five DNA polymerases all of which are responsible for nuclear DNA replication.

4. Since the rate of form movement in eukaryotes is much slower than that of bacteria, chromosomes require more than an hour to replicate.

5. Although histones and DNA are made in the same parts of the eukaryotic cell, the packaging of them in the nucleosome slows down replication so that it is slower than that of prokaryotes.

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#Question id: 15617

#Unit 13. Methods in Biology

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition you have a wild type (Lac+) strain carrying a Tn5 insertion known to be near several Lac genes on the E. coli chromosome. You grow P1 phage on this strain and use the resulting phage lysate to infect the lac1– strain, selecting for kanamycin resistance (Kanr). Among 100 Kanr transductants, you find that 82 are Lac– and 18 are Lac+. Express the distance between Tn5 and the lac1– mutation as a cotransduction frequency;

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#Question id: 19702

#Unit 13. Methods in Biology

What is aVL in augmented unipolar limb lead?

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#Question id: 30118

#Unit 1. Molecules and their Interaction Relevant to Biology

The following enzymes are involved in the catabolism of AMP to uric acid. The correct order of their use is:

1. A deaminase

2. A nucleoside phosphorylase

3. A nucleotidase

4. Xanthine oxidase

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#Question id: 20000

#Unit 13. Methods in Biology

Example of Cross-reactivity

a) Dominant and recessive gene

b) Autoimmune disease

c) Blood group mismatch

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#Question id: 32520

#Unit 1. Molecules and their Interaction Relevant to Biology

Match the following