TLS Online TPP Program

#Question id: 3837


How does the enzyme telomerase meet the challenge of replicating the ends of linear chromosomes?

#Unit 3. Fundamental Processes
  1. It adds a single 5' cap structure that resists degradation by nucleases.

  2. It causes specific double-strand DNA breaks that result in blunt ends on both strands.

  3. It catalyzes the lengthening of telomeres, compensating for the shortening that could occur during replication without telomerase activity.

  4. It adds numerous GC pairs, which resist hydrolysis and maintain chromosome integrity.

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TLS Online TPP Program

#Question id: 4403

#Unit 3. Fundamental Processes

 Which of the following does (do) not require a DNA helicase activity?

a. SWI/SNF function  b. Pol II open-complex formation

c. transcription-factor binding to DNA

d. deacetylation of histone tails

TLS Online TPP Program

#Question id: 4404

#Unit 3. Fundamental Processes

The yeast two-hybrid system can be used to identify

a. proteins that interact with a known or “bait” protein.

b. proteins that interact with a “fish” domain.

c. cDNAs that encode interacting proteins.

d. co-activators and co-repressors.

TLS Online TPP Program

#Question id: 4405

#Unit 3. Fundamental Processes

Which of the following can occur when steroid hormone binds to its cognate receptor?

a. conformational changes in the receptor

b. translocation of the receptor out of the cell membrane

c. binding of the receptor to DNA response elements

d. stimulation of transcription

TLS Online TPP Program

#Question id: 4406

#Unit 3. Fundamental Processes

An electrophoretic mobility shift assay (EMSA) was conducted to check for the binding of TFIIB to a segment of a DNA promoter region. Which of the following approaches could be used to confirm that TFIIB is indeed binding?

TLS Online TPP Program

#Question id: 4407

#Unit 3. Fundamental Processes

The general transcriptional factors are used by all sets of RNA Polymerase. Which of the following GTF is/are commonly used by all RNA Polymerases?

A. TBP

B. TFIID

C. TFIIIC

D.TFIIE

E. TFIIH

TLS Online TPP Program

#Question id: 4408

#Unit 3. Fundamental Processes

an example/s of in vivo “molecular mimicry” is/are

I. EF-G–GDP and EF-Tu–GTP–tRNA

II. TAF42 -TAF62 and H3.H4 tetramer

III. Tyrosinyl-tRNA and Puromycin