TLS Online TPP Program

#Question id: 3846


A heat-sensitive DNA ligase (25°C permissive, 37°C nonpermissive) has been used to show that DNA synthesis is discontinuous. Examination of DNA replication in the presence of [3H]-thymidine in the mutant would demonstrate which of following?

#Unit 3. Fundamental Processes
  1. The accumulation of short segments of unlabeled DNA at 25°C and at 37°C

  2. The accumulation of short segments of unlabeled DNA at 25°C but not at 37°C

  3. The accumulation of short segments of radioactive DNA at 25°C but not at 37°C

  4. The accumulation of short segments of radioactive DNA at 37°C but not at 25°C