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TLS Online TPP Program

#Question id: 3864

RNA polymerase:

#Unit 3. Fundamental Processes

  1. binds tightly to a region of DNA thousands of base pairs away from the DNA to be transcribed.

  2. can synthesize RNA chains de novo (without a primer).

  3. has a subunit called lambda, which acts as a proofreading ribonuclease.

  4. separates DNA strands throughout a long region of DNA (up to thousands of base pairs), then copies one of them.

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TLS Online TPP Program

#Question id: 13065

#Unit 13. Methods in Biology

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Agglutination of antigens by utilising specific antibodies can only occur, if
TLS Online TPP Program

#Question id: 13066

#Unit 13. Methods in Biology

Precision will be reduced, but yield will be increased Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following is a codominant marker?
TLS Online TPP Program

#Question id: 13067

#Unit 13. Methods in Biology

The first immunoassay formats described were methods based on the ____ reaction, which is characterised either by gel formation in a liquid phase or as an opaque band in an agar plate assay.
TLS Online TPP Program

#Question id: 13068

#Unit 13. Methods in Biology

Coating antibody; which actively traps antigen
TLS Online TPP Program

#Question id: 13069

#Unit 13. Methods in Biology

A negative agglutination test may NOT indicate the
TLS Online TPP Program

#Question id: 13070

#Unit 13. Methods in Biology

Antibodies raised against an antigen from one organism will react to a greater or lesser degree with similar antigens from a related organism, depending on how many surface epitopes they share. This has given rise to a systematic method of identification of microorganisms known as______?