TLS Online TPP Program

#Question id: 24469


Which of the following statement is incorrect 

#Unit 3. Fundamental Processes
  1. Molecular genetic techniques are used to make a cDNA encoding a recombinant protein to which is fused a short sequence of amino acids called an epitope tagging.
  2. Two commonly used epitope tags are FLAG, which encodes the amino acid sequence DYKDDDDK   (single- letter amino acid code), and myc, which encodes the sequence EQKLISEEDL.
  3. Two commonly used epitope tags are myc, which encodes the  amino acid  sequence DYKDDDDK   (single- letter amino  acid code), and FLAG, which encodes the sequence EQKLISEEDL.
  4. RNA Polymerase can also separated by using epitope tagging. Normally RPB3 is used to create epitope tag
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TLS Online TPP Program

#Question id: 3824

#Unit 3. Fundamental Processes

State whether the following statements is true/false regarding DNA replication.

1. The polymerization of DNA is essentially irreversible due to the hydrolysis of pyrophosphate.

2. On average about twenty errors are made during each replication of the human genome.

3. DNA polymerase I possesses 5ʹ → 3ʹ exonuclease activity.

4. All DNA ligase enzymes use ATP as a cosubstrate.

5. The purpose of SSB is to prevent single-stranded DNA from folding back on itself to form double-helical regions.

TLS Online TPP Program

#Question id: 3825

#Unit 3. Fundamental Processes

Telomeres are the repetitive sequence of T's and G's that are present in the eukaryotes to protect the random cleavage from nucleases. These telomeres are synthesized by the enzyme telomerase. Which of the following are the properties of enzyme telomerase?

TLS Online TPP Program

#Question id: 3826

#Unit 3. Fundamental Processes

Which scientists first gave experimental evidence that DNA is the genetic material?

TLS Online TPP Program

#Question id: 3827

#Unit 3. Fundamental Processes

It takes about the same amount of time for eukaryotic chromosomes to be replicated in vivo as it does for E. coli. Why is this true?

TLS Online TPP Program

#Question id: 3828

#Unit 3. Fundamental Processes

Why does E. coli need both DNA polymerase III and DNA polymerase I?

TLS Online TPP Program

#Question id: 3829

#Unit 3. Fundamental Processes

Why is only one copy of DNA made per cell division cycle in eukaryotes despite the presence of multiple origins of replication?