Splice sites are directional in nature. 5’ splice site -the left, or donor, site and the 3’ splice site - right, or acceptor, site. 

1. U2 addition to base pair with the branch site in the presence of ATP

2. U1 and U4 release Formation of the catalytic center in which U6 base pairs with the 5' splice site; U6 also base pairs with U2; U2 remains base paired with the branch site; U5 interacts with both exons through its loop.

So, 1 and 2 are the event of complex formation which are 

#Question id: 648

#Unit 1. Molecules and their Interaction Relevant to Biology

Enzyme catalysis is regulated by a number of mechanisms. Also, the flux through metabolic pathways must be regulated. Given the metabolic pathway below, which choice accurately describes the regulation of compound G levels?

#Question id: 649

#Unit 1. Molecules and their Interaction Relevant to Biology

Choose INCORRECT statement from the following

#Question id: 650

#Unit 1. Molecules and their Interaction Relevant to Biology

On the basis of hypothetical binding curve given below, which of the following statement is INCORRECT

#Question id: 651

#Unit 1. Molecules and their Interaction Relevant to Biology

Consider an enzyme catalysed reaction treated with inhibitor. Enzyme kinetic was analysed with Eadie Hofstee equation. A plot of V0 versus V0/[S] for an enzyme-catalyzed reaction is shown below. The control curve was obtained in the absence of inhibitor. Which effect is shown on the enzyme activity when an inhibitor is added to the reaction mixture?

#Question id: 652

#Unit 1. Molecules and their Interaction Relevant to Biology

Two proteins, X and Y, bind to the same ligand, A, with the binding curves shown below. Which of the statement is correct for the graph analysing two proteins?

#Question id: 653

#Unit 1. Molecules and their Interaction Relevant to Biology

For the enzyme-catalyzed reaction S ~ P, Keq = 5, which of the following curves best represents the change in [S] that occurs with time when S is added to a solution containing the appropriate enzyme?