TLS Online TPP Program

#Question id: 26802


The exchange of bacterial genes clearly required direct contact, that is given by

#Unit 8. Inheritance Biology
  1. Davi’s
  2. Lederberg 
  3. Tatum’s
  4. Lederberg & Tatum’s
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TLS Online TPP Program

#Question id: 13082

#Unit 13. Methods in Biology

Statement: AFLP shares some features of both RFLP and RAPD analyses.
Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.
II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.

TLS Online TPP Program

#Question id: 13083

#Unit 13. Methods in Biology

The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments: 
(1) both ends cleaved by PstI (Pst-Pst), 
(2) both ends cleaved by MseI (Mse-Mse), and 
(3) one end generated by each of the two enzymes (Pst-Mse). 
the most frequent fragments in decreasing order,

TLS Online TPP Program

#Question id: 13084

#Unit 13. Methods in Biology

Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.
Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.
II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

TLS Online TPP Program

#Question id: 13085

#Unit 13. Methods in Biology

The most common microsatellite sequence encountered in human genome is the sequence 

TLS Online TPP Program

#Question id: 13086

#Unit 13. Methods in Biology

Which group of VNTRs are tandem are frequently distributed in all eukaryotic genomes (except yeast) examined so far. They show a large, stable 'polymorphism due to variation in the number of repeat units and are almost 'ideal as molecular markers for genome mapping.

TLS Online TPP Program

#Question id: 13087

#Unit 13. Methods in Biology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              
Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?