An example of coordinate control is the down-regulation of ribosomal RNA synthesis in response to amino acid starvation, which will cause synthesis of ribosomal proteins to be limited.  What is the correct order of the following events that participate in the signaling process?

1. Binding of stringent factor to the ribosome.

2. Formation of the unusual nucleotide ppGpp.

3. Formation of the unusual nucleotide pppGpp.

4. Uncharged tRNA binds in the ribosomal A-site.

TLS Online TPP Program

#Question id: 12917

#Unit 13. Methods in Biology

Arrange the steps of stages in PCR in correct order.

1. The region to be specifically amplified is made accessible within the complex DNA.

2. The double stranded DNA is denatured by heating the reaction mixture to above 90 degree Celsius.

3. A thermal cycler is used to incrementally decrease the annealing temperature.

4. Reaction mixture is then cooled to a temperature between 40 and 60 degree celsius.

TLS Online TPP Program

#Question id: 12918

#Unit 13. Methods in Biology

Name the enzyme which helps in the DNA synthesis in the PCR.

TLS Online TPP Program

#Question id: 12919

#Unit 13. Methods in Biology

Which of the following statement is not correct about RT-PCR. 

TLS Online TPP Program

#Question id: 12920

#Unit 13. Methods in Biology

The development of isothermal systems such as the ____ DNA amplification system do away with the need for a thermal cycler and have the advantage of being able to be used outside the laboratory.

TLS Online TPP Program

#Question id: 12921

#Unit 13. Methods in Biology

Match the following:-

A. Gene probe production.	i. Novel proteins
B. Genome mapping.	ii. PCR mutagenesis
C. Protein Engineering.	iii. Sequence tagged sites
D. Gene Editing.	iv. Use with blots

TLS Online TPP Program

#Question id: 12922

#Unit 13. Methods in Biology

 Match the following:-

1. Ligase chain reaction.	i. Mutation detection
2. Nucleic acid sequence based amplification.	ii. Non isothermal
3. Loop mediated isothermal amplification.	iii. Viral detection
4. Branched DNA amplification.	iv. parasitic disease