You are studying the effects of α-amanitin, a poisonous cyclic octapeptide, on eukaryotic cells and have noticed that following treatment, there is no miRNA transcription. Based on this evidence you conclude that α-amanitin must be inhibiting:

TLS Online TPP Program

#Question id: 18709

#Unit 13. Methods in Biology

qPCR can be done by 

i) cyanin dye or SYBR green – to measure amplification

ii) Taqman assay – measure extension

iii) molecular beacons - denaturation

TLS Online TPP Program

#Question id: 18710

#Unit 13. Methods in Biology

qPCR  graph is drawn between ∆Rn and PCR cycle In this graph CT represents

 

TLS Online TPP Program

#Question id: 18711

#Unit 13. Methods in Biology

SYBR green qPCR based on 

TLS Online TPP Program

#Question id: 18712

#Unit 13. Methods in Biology

which of the following are two major functions of RE

TLS Online TPP Program

#Question id: 18713

#Unit 13. Methods in Biology

How many DNA duplex is obtained from one DNA duplex after 4 cycles of PCR?

TLS Online TPP Program

#Question id: 18714

#Unit 13. Methods in Biology

At what temperature do denaturation of DNA double helix takes place?