In a biochemical experiment, you compare the products from splicing reactions carried out in vitro using three different substrates. In each case the substrate is a construct containing a single intron surrounded by two exons, and in all cases the construct is the same overall size. But in one case, the intron is a group I intron, in another a group II intron, and in the third an intron removed by the spliceosome. Each construct is labelled in a manner that allows it to be detected after gel electrophoresis, and each is tested in two reactions—one, conditions that support self-splicing, and two, in the presence of nuclear extract as well. Note that, for simplification, only the final products of the splicing reaction are seen, but before degradation of the introns.

 Which of the following observation is/are correct?

A. In lane A, the presence of nuclear abstract, a band for the lariat and the spliced product and pre-mRNA.

B. Lane B shows Group I Introns and Lane C is for Group II introns and reaction is nonself-splicing

C. Lane C is Group I intron. Splicing takes place in the absence and presence of nuclear abstract, and the reaction is self-splicing.

#Question id: 7305

#Unit 5. Developmental Biology

In plants, a growing mass of unorganized and undifferentiated cells that cover a wound; these cells can be induced to form a plant meristem and develop into shoots and/or roots called as

#Question id: 7306

#Unit 5. Developmental Biology

A plant scientist was hired by a greenhouse operator to devise a way to force iris plants to bloom in the short days of winter. Iris normally blooms as a long-day (short-night) plant. Which of the following has the best chance of creating iris blooms in winter?

#Question id: 7307

#Unit 5. Developmental Biology

Which of the following statements regarding flowering plants is correct?

#Question id: 7308

#Unit 5. Developmental Biology

In Arabidopsis thaliana, the transition to flowering involves multiple factors, pathways and meristem identity genes. Following statements regarding to the mechanism and function of meristem identity genes.

 A. FT mRNA is expressed in companion cells of the leaf vein in response to multiple signals, including day length, light quality, and temperature.

 B. The FT–FD complex activates AP1 in the inflorescence meristem and SOC1 in the floral meristem, which triggers LFY gene expression.

 C. LFY and AP1 trigger expression of the floral homeotic genes.

 D. FLC acts as a negative regulator of SOC1 in the meristem and as a negative regulator of FT in the leaves.

 Which of the following is incorrect?

#Question id: 7309

#Unit 5. Developmental Biology

Following statements are regarding to the molecular basis of the coincidence model in Arabidopsis.

A. In Arabidopsis under short days, there is little overlap between CO mRNA expression and daylight.

B. CO protein does not accumulate to sufficient levels in the phloem to promote the expression of the transmissible floral stimulus and the plant remains vegetative.

C. Under long days, the peak of CO mRNA abundance (at hours 12 through 16) overlaps with daylight (sensed by phyA and cryptochrome [cry]), allowing CO protein to accumulate.

D. CO activates FLC mRNA expression in the phloem, which causes flowering when the FT protein is translocated to the apical meristem.

Which of the following is incorrect?

#Question id: 7310

#Unit 5. Developmental Biology

Which statement best describes the mechanism for circadian rhythm?