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TLS Online TPP Program
#Question id: 13055
#Unit 13. Methods in Biology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following will provide least specific amplification in qPCR?
TLS Online TPP Program
#Question id: 4527
#Unit 3. Fundamental Processes
The Mediator complex
TLS Online TPP Program
#Question id: 28269
#Unit 5. Developmental Biology
Sperm-specific calcium channels are encoded by which gene?
TLS Online TPP Program
#Question id: 19046
#Unit 13. Methods in Biology
Which of the following techniques is used to study the three-dimensional structure of a molecule?
TLS Online TPP Program
#Question id: 10578
#Unit 10. Ecological Principles
The population of annual breeder growing at a rate of 10% per year. Initial population size is 1200. After 3 years population size would be