Nitrogen (N) normally forms three covalent bonds with a valence of five. However, ammonium has four covalent bonds, each to a different hydrogen (H) atom (H has a valence of one). What do you predict to be the charge on ammonium?

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#Question id: 23157

#Unit 13. Methods in Biology

Immunofluorescence microscopy, the most widely used method of detecting 

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#Question id: 23158

#Unit 13. Methods in Biology

You have purified protein X and injected into an animal so that over a period of weeks, the animal will mount an immune response and make antibodies to protein X. By which technique these generated antibodies can be extracted from blood serum.

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#Question id: 23159

#Unit 13. Methods in Biology

Rhodamine is a fluorescent dye which emits a colour?

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#Question id: 23160

#Unit 13. Methods in Biology

Texas is a fluorescent dye which emits a colour?

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#Question id: 23161

#Unit 13. Methods in Biology

A classically used fluorescent dye which emits a orange light is?

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#Question id: 23162

#Unit 13. Methods in Biology

Match the fluorescent dyes in column I with emission light in column II

Column I                                                                          Column II

1. Texas                                                                            a. far red

2. Fluorescein                                                                   b. red

3. Rhodamine                                                                   c. blue 

4. Cy5                                                                               d. green

Which of the following option comprises all correct matches?