Promoter consensus sequence was derived by aligning 300 sequences known to function as s70 promoters and ascertaining the most common base found at each position in the –35 and –10 hexamers are,

#Question id: 4394

#Unit 3. Fundamental Processes

A new insulin gene was isolated and a study was planned to look at the function of the promoter region. Which of the following would be the best choice for this study?

A. southern analysis

B. immunoblotting

C. reporter gene assay

D. PCR

#Question id: 4395

#Unit 3. Fundamental Processes

Which of the following chemicals would be the most appropriate for the 5’ labeling of a DNA strand with a radioactive phosphate?

#Question id: 4396

#Unit 3. Fundamental Processes

It is suspected that a novel protein only interacts with the promoter region of a gene. Which of the following techniques should be used to confirm this suspicion?

A. EMSA

B. DNase footprinting

C. Filter binding

D. Gel mobility shift assay

#Question id: 4397

#Unit 3. Fundamental Processes

Which of the following will prevent the activation of RNA polymerase, but not necessarily the assembly of eukaryotic general transcription factors at the promoter?

#Question id: 4398

#Unit 3. Fundamental Processes

Which of the following are not found in DNA-binding proteins?

#Question id: 4399

#Unit 3. Fundamental Processes

Transcription factors can

a. exhibit cooperative binding.  b. exist as heterodimers.

c. act to repress transcription of transcription factor genes.

d. undergo conformational changes which alter activity.

e. never interact with co-repressors.