Choose correct statements about recognition mechanism of TBP ;

A. TBP causes the minor groove to be widened to an almost flat conformation

B. It also bends the DNA by an angle of ~120o

C. The interaction between TBP and DNA involves maximum number of hydrogen bonds between the protein and the edges of the base.

D. Much of the specificity is imposed by two pairs of phenylalanine side chains that intercalate between the base pairs at either end of the recognition sequence and drive the strong bend in the DNA.

TLS Online TPP Program

#Question id: 33985

#Unit 2. Cellular Organization

To analyze existing MARs that are bound to the matrix in vivo, what is correct order of method 

A. Chromosomal loops decondensed by extracting the chromatin proteins. 

B. Removal of the DNA loops by treatment with restriction nucleases

C. Amplification of MAR and sequencing

TLS Online TPP Program

#Question id: 33986

#Unit 2. Cellular Organization

What is the relationship between the chromosome scaffold of dividing cells and the matrix of interphase cells

A. The same DNA fragments that are found within the nuclear matrix in vivo can be retrieved from the metaphase scaffold

B. Fragments containing MAR sequences can bind to a metaphase scaffold; it is likely that DNA contains a single type of attachment site.

C. In interphase cells the attachment site is connected to the nuclear matrix, whereas in mitotic cells it is connected to the chromosome scaffold.

TLS Online TPP Program

#Question id: 34086

#Unit 3. Fundamental Processes

Replicators/replicons can be identified using a 

TLS Online TPP Program

#Question id: 34087

#Unit 3. Fundamental Processes

A DNA containing which an origin of replication undergoing to initiation of replication and further, is digested with restriction endonuclease. Which shape of DNA segment can be found after digestion followed electrophoresis.

A. Y Shape B. Bubble shape C. bubble-to-Y-arc D. Circular

TLS Online TPP Program

#Question id: 34088

#Unit 3. Fundamental Processes

How can the 2D gels identify the DNA intermediates associated with a replication origin? Choose combination of correct statements

A. Y-shaped molecule that has three equal length arms will migrate the most slowly of all such molecules derived from a particular DNA fragment

B. A Y-shaped molecule with two very short replicated arms and a large replicated region will migrate very similarly to the unreplicated version of the same DNA fragment. 

C. Y-shaped molecule that results from the almost completely replicated fragment is similar in shape to a linear molecule two times the size of the unreplicated fragment.

TLS Online TPP Program

#Question id: 34089

#Unit 3. Fundamental Processes

A. Replication Initiation require full methylation of the Dam target sites in the origin

B. Replication Initiation inhibited by hemimethylation of Dam target sites 

C. Replication Initiation inhibited by nonmethylation of Dam target sites 

D. An origin of nonmethylated DNA can function effectively

Choose true and false