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TLS Online TPP Program

#Question id: 4511

The proteins are not needed for the splicing reaction itself, because

#Unit 3. Fundamental Processes

  1. In vivo, the intron is complexed with several proteins that help stabilize the correct structure-partly by shielding regions of the backbone from each other.

  2. The negative charges provided by the phosphates in those backbone regions would repel each other if shielded.

  3. In vitro, high salt concentrations (and thus positive ions) compensate for the absence of these proteins.

  4. Only a and d

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TLS Online TPP Program

#Question id: 18959

#Unit 13. Methods in Biology

 Vectors used for construction of genomic libraries, and their capacity is given match them correctly

A- λ EMBL seriesI - 300kb - 1,400 kb
B - CosmidsII - 45 kb
C - BACs and YACs (high capacity vectors)III - 20kb
TLS Online TPP Program

#Question id: 18960

#Unit 13. Methods in Biology

cDNA library do not require very large vectors hence which of the following is commonly used in cDNA library
TLS Online TPP Program

#Question id: 18961

#Unit 13. Methods in Biology

When a cDNA library is generated by first removing those mRNA or cDNA sequences that are common to two sources, e.g., two different Cell types, such a library is called 
TLS Online TPP Program

#Question id: 18962

#Unit 13. Methods in Biology

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA,

2. Their large size makes the handling procedures during cloning tedious and cumbersome

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned.

These three problems have been resolved by deleting 
TLS Online TPP Program

#Question id: 18963

#Unit 13. Methods in Biology

The use of wild type pTi as a vector presents the following three problems.
1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA, which causes a disorganised growth and a loss of regeneration potentiaLof the cells having T-DNA in their genomes.
2. Their large size makes the handling procedures during cloning tedious and cumbersome.
3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned
In the above mention’s problems, 1 and 3 can removed by disarming and creating MCS, to overcome from 2nd problem need to develop
TLS Online TPP Program

#Question id: 18964

#Unit 13. Methods in Biology

Size of agrobacterium can be reduced by binary vector formation; these binary vectors can be formed by phenomenon of