The activators bind—and the enhanceosome forms—in a highly cooperative manner, ensuring that all three activators must be present. The following are three ways the regulators might be binding cooperatively:

I- Through direct protein–protein interactions between them

II- By changes in the DNA caused by binding of one protein helping binding of another;

III- By the fact that the activators all interact simultaneously with the coactivator.

TLS Online TPP Program

#Question id: 4445

#Unit 3. Fundamental Processes

The active site, which is made up of RPB1 and RPB2, from both these subunits, is found at the base of the pincers within a region called the “active center cleft” . The active site works according to the two-metal ion catalytic mechanism for nucleotide addition proposed for all types of polymerase;

TLS Online TPP Program

#Question id: 4446

#Unit 3. Fundamental Processes

Transcription start site is designated with the;

TLS Online TPP Program

#Question id: 4447

#Unit 3. Fundamental Processes

Sequences in the direction in which transcription proceeds are referred to as __?from the start site, and allotted a? value.

TLS Online TPP Program

#Question id: 4448

#Unit 3. Fundamental Processes

Three defined steps in a transcription cycle; choose correct one

1a. Closed complex	In this form, the DNA remains double-stranded, and the enzyme is bound to one face of the helix. I
1b. Open complex	In which the DNA strands separate over a distance of 13 bp around the start site to form the transcription bubble. In the next stage of initiation,
2. Promoter escape	Incorporation of the first 10 or so ribonucleotides is a rather inefficient process, and at that stage, the enzyme often releases short transcripts and then begins synthesis again
3. Initial transcribing complex.	Once an enzyme makes a transcript longer than 10 nucleotides, it is said to have

TLS Online TPP Program

#Question id: 4449

#Unit 3. Fundamental Processes

Promoters recognized by polymerase containing s70 share the following characteristic structure;

TLS Online TPP Program

#Question id: 4450

#Unit 3. Fundamental Processes

According to the correlation between promoter strength and sequence, in all these promoters which one is seems like very weak promoter or non-existing in any organism