The treeʹs horizontal axis is a timeline that extends from 100,000 years ago to the present; the vertical axis represents nothing in particular. The labeled branch points on the tree (V—Z) represent various common ancestors. Letʹs say that only since 50,000 years ago has there been enough variation between the lineages depicted here to separate them into distinct species, and only the tips of the lineages on this tree represent distinct species.

According to this tree, what percent of the species seem to be extant (i.e., not extinct)?

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#Question id: 23151

#Unit 13. Methods in Biology

If the concentration of Ca2+ is high inside the cell and fura-2 dye is used to map the concentration which of the following conditions will prevail?

1. Fluorescence will decrease

2.  Fluorescence will increase

3. Emission of light at higher wavelength

4. Emission of light at lower wavelength

Which of the following options are correct?

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#Question id: 23152

#Unit 13. Methods in Biology

A fluorescent dye SNARF-1 that is sensitive to H+ concentrations can be used to monitor the cytosolic pH of 

1. Alkaline cells

2. Acidic cells 

3. Dead cells

4. Live cells

Which of the following options are incorrect?

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#Question id: 23153

#Unit 13. Methods in Biology

SNARF-1is a fluorescent dye used to measure which ion concentration?

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#Question id: 23154

#Unit 13. Methods in Biology

When the organelles pH is acidic the how the SNARF-1 dye measures this acidity?

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#Question id: 23155

#Unit 13. Methods in Biology

Match the following ions in column I with their relevant dyes column II.

Column I                                                       Column II

1. K+                                                                 a. Fura-2

2. Ca2+                                                             b. SNARF-1

3. H+                                                                 c. Ion potassium green

Which of the following options is correct?

TLS Online TPP Program

#Question id: 23156

#Unit 13. Methods in Biology

Most widely used method of detecting specific proteins, uses an antibody to which a fluorescent dye has been covalently attached