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TLS Online TPP Program

#Question id: 5128

During lens formation in the Xenopus, the following statements have been proposed. Which statement is incorrect?

#Unit 5. Developmental Biology

  1. Expression of Pax6 protein may constitute the competence of the surface ectoderm to respond to the optic vesicle during the late neurula stage.

  2. The optic vesicle secretes BMP and FGF family paracrine factors that induce the synthesis of the Sox transcription factors and initiate lens formation.

  3. If Pax6 is lost, whether it is in fruit flies, frogs, rats, or humans, it results in a complete loss or reduction of the eyes.

  4. If Pax6 is lost, still lens formation takes place in presence of Sox2 and L-maf.

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TLS Online TPP Program

#Question id: 12916

#Unit 13. Methods in Biology

Which statement is correct for Zoo blotting?
TLS Online TPP Program

#Question id: 12917

#Unit 13. Methods in Biology

Arrange the steps of stages in PCR in correct order.
1. The region to be specifically amplified is made accessible within the complex DNA.
2. The double stranded DNA is denatured by heating the reaction mixture to above 90 degree Celsius.
3. A thermal cycler is used to incrementally decrease the annealing temperature.
4. Reaction mixture is then cooled to a temperature between 40 and 60 degree celsius.
TLS Online TPP Program

#Question id: 12918

#Unit 13. Methods in Biology

Name the enzyme which helps in the DNA synthesis in the PCR.
TLS Online TPP Program

#Question id: 12919

#Unit 13. Methods in Biology

Which of the following statement is not correct about RT-PCR. 
TLS Online TPP Program

#Question id: 12920

#Unit 13. Methods in Biology

The development of isothermal systems such as the ____ DNA amplification system do away with the need for a thermal cycler and have the advantage of being able to be used outside the laboratory.
TLS Online TPP Program

#Question id: 12921

#Unit 13. Methods in Biology

Match the following:-
A. Gene probe production.              i. Novel proteins
B. Genome mapping.                       ii. PCR mutagenesis
C. Protein Engineering.                 iii. Sequence tagged sites
D. Gene Editing.                                iv. Use with blots