A generalized transducing phage is used to transduce an a- b- c- d- e- recipient strain of E. coli with an a+ b+c+d+ e+ donor. The recipient culture is plated on various media with the results shown in the table below What can you conclude about the linkage and order of the genes?

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#Question id: 12907

#Unit 13. Methods in Biology

The binding of a regulatory protein or transcription factor to a specific DNA site results in a complex which may be analyzed by the technique termed as _____

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#Question id: 12908

#Unit 13. Methods in Biology

Which statement is incorrect about CRISPR?

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#Question id: 12909

#Unit 13. Methods in Biology

According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

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#Question id: 12910

#Unit 13. Methods in Biology

Match the following:

A. RNA analysis	i. Sexing or cell mutation sites	a. Expressed Sequence tag
B. Forensic science	ii. mRNA analysis	b. Analysis of DNA from blood
C. Gene discovery	iii. DNA profiling	c. Active latent viral infections
D. Single-cell analysis	iv. RT–PCR	d. Sex determination of unborn

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#Question id: 12911

#Unit 13. Methods in Biology

Match the following:

A. DNA polymerases	i. Alkaline phosphatase	a. RNA-dependent DNA polymerase, used in cDNA synthesis
B. Reverse transcriptase	ii. T4 DNA ligase	b. Thermostable DNA polymerase used in PCR
C. Ligases	iii. AMV-RT	c. Removes 5'-phosphates from DNA and RNA
D. Phosphatases	iv. Taq DNA pol	d. Links 5'-phosphate and 3'-hydroxyl ends via phosphodiester bond

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#Question id: 12912

#Unit 13. Methods in Biology

Name the enzyme which separates the double-helical DNA into single strands.