TLS Online TPP Program

#Question id: 5301


Which of the following processes is an early step in the whole-genome shotgun approach to sequencing?

#Unit 11. Evolution and Behavior
  1. break genomic DNA at random sites

  2. map the position of cloned DNA fragments

  3. randomly select DNA primers and hybridize these to random positions of chromosomes in preparation for sequencing

  4. break genomic DNA at specific sites

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TLS Online TPP Program

#Question id: 11809

#Unit 6. System Physiology – Plant

Shortly after the discovery of kinetin, it was observed that the differentiation of cultured callus tissue derived from tobacco pith segments into either roots or shoots depends on the ratio of auxin to cytokinin in the culture medium. Whereas

a) high auxin:cytokinin ratios stimulated the formation of roots

b) high auxin:cytokinin ratios stimulated the formation of shoots

c) low auxin:cytokinin ratios led to the formation of shoots

d) low auxin:cytokinin ratios stimulated the formation of roots

which of the following combination is correct about the ratio of auxin to cytokinin

TLS Online TPP Program

#Question id: 11810

#Unit 6. System Physiology – Plant

An experiment was done to see the effect of auxin: cytokinin ratios on morphogenesis can also be seen in crown gall tumors by mutation of the T-DNA of the Agrobacterium Ti plasmid,

I) Mutating the ipt gene (the tmr locus) of the Ti plasmid blocks zeatin biosynthesis in the infected cells

II) mutating either of the genes for auxin biosynthesis (tms locus)

Given some results about these above mutations ,

a) equal auxin to cytokinin ratio form undifferentiated callus tissue

b) high auxin:cytokinin ratio in the tumor cells causes the proliferation of roots instead of undifferentiated callus tissue

c) lowers the auxin:cytokinin ratio and stimulates the proliferation of shoots

What would be the result of these two mutation ?

TLS Online TPP Program

#Question id: 11811

#Unit 6. System Physiology – Plant

The expression of  IPT gene in the stress is driven by___

TLS Online TPP Program

#Question id: 11812

#Unit 6. System Physiology – Plant

The  nitrogens  at the 3, 7, and 9 positions of the adenine ring of cytokinins can be conjugated to glucose residues, Alanine can also be conjugated to the nitrogen at the 9 positon, forming

TLS Online TPP Program

#Question id: 11813

#Unit 6. System Physiology – Plant

Cytokinin-overproducing plants exhibit several characteristics that point to roles played by cytokinin in plant physiology and development:

a) shoot apical meristems of cytokinin-overproducing plants produce more leaves.

b) Adventitious shoots may form from unwounded leaf veins and petioles.

c) Leaf senescence is retarded.

d) more extreme cytokinin-overproducing plants are stunted, with greatly shortened internodes.

e) Apical dominance is greatly increased

Which of the following statements of above given overproducing cytokinin plants is correct?

TLS Online TPP Program

#Question id: 11814

#Unit 6. System Physiology – Plant

Simplified biosynthetic pathway for cytokinin biosynthesis followed given such steps;

a) isopentenyl transferase (IPT), is the addition of the isopentenyl side chain from DMAPP (dimethylallyl diphosphate) to an adenosine moiety (AMP)

b) iPRTP or iPRDP is converted to ZTP or ZDP respectively by cytochrome P450 monooxygenase  and eventually is converted to zeatin

c) The ribotide and riboside forms of trans-zeatin can be interconverted, and free transzeatin can be formed from the ribotide by the LONELY GUY (LOG) family of cytokinin

Which of the following steps is true of the cytokinin biosynthesis?