TLS Online TPP Program

#Question id: 5656


What is the cortical reaction, and why is it important?

#Unit 5. Developmental Biology
  1. The cortical reaction is the entry of Ca2+ ions into the egg, which initiates development.

  2. The cortical reaction is the exocytosis of the cortical granules during egg maturation, which contain the components of the zona pellucida.

  3. The cortical reaction is the depolarization of the plasma membrane after sperm entry, which helps to block polyspermy.

  4. The cortical reaction is the release of the cortical granules after sperm entry, which converts the vitelline membrane into the fertilization membrane which blocks polyspermy.

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TLS Online TPP Program

#Question id: 18676

#Unit 13. Methods in Biology

which of the following technique can be analysed hybridisation probes,  gene-cloning use as primers for DNA sequencing,  the polymerase chain reaction (PCR), use in site-directed mutagenesis and use as antisense therapeutics

TLS Online TPP Program

#Question id: 18677

#Unit 13. Methods in Biology

Point mutations in DNA, such as occur in a range of human diseases, can be identified by 

TLS Online TPP Program

#Question id: 18678

#Unit 13. Methods in Biology

which of the following technique can be analysed of PCR products from HIV-I allowed the identification of between 200 000 and 500 000 viral particles per cubic centimetre of serum.

TLS Online TPP Program

#Question id: 18679

#Unit 13. Methods in Biology

Chiral compounds can be resolved using

TLS Online TPP Program

#Question id: 18680

#Unit 13. Methods in Biology

DNA from Salmonella species, when digested with the restriction enzyme Xba I, gives around 15 fragments ranging from 25 kb to 680 kb This pattern of fragments, or ‘fingerprint’, is unique to that strain. If the same fingerprint is found from bacteria from other infected people, then it can be assumed that they were all infected from a common source. Thus, by comparing their eating habits, food sources, etc. the source of the infection can be traced to a restaurant, food item. The fingerprints are created and analysed by

TLS Online TPP Program

#Question id: 18681

#Unit 13. Methods in Biology

Highly heterogeneous mixtures of proteins such as cell lysates or tissue extracts should be completely fractionated into individual proteins so as to obtain an overall picture of the protein composition and to enable location of individual proteins. The highest resolution is obtained