Given below, is the result of a complementation test for six independent mutants (1 to 6)

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#Question id: 34170

#Unit 3. Fundamental Processes

What is correct about Klenow fragment

A. is a single polypeptide of 103 kD

B. larger cleavage product of DNA Pol I of 68 kD

C. It contains the polymerase activity

D. It contains the 3’-5’ exonuclease activity

E. It contains the 5’-3’ exonuclease activity

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#Question id: 34171

#Unit 3. Fundamental Processes

If the rate of fidelity of replication in bacteria is 10^-9/bp. How many replications it would take to make a mistake?

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#Question id: 34172

#Unit 3. Fundamental Processes

Frameshifts occur when an extra nucleotide is inserted or omitted. Which of sequence and property is most sensitive combination for the frameshift to occur

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#Question id: 34173

#Unit 3. Fundamental Processes

The 3’–5’ exonucleolytic activity is found in which subunit of bacterial DNA Polymerase III

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#Question id: 34174

#Unit 3. Fundamental Processes

Once bound to a primer:template junction, DNA polymerase interacts tightly with much of the double stranded portion of the DNA by

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#Question id: 34175

#Unit 3. Fundamental Processes

A. For processive DNA synthesis, proofreading occurs without releasing the DNA from the polymerase

B. When a mismatched base pair is present in the polymerase active site, the primer:template junction is destabilize

C. The DNA polymerase active site binds such a mismatched template poorly, but the exonuclease active site has a 10-fold higher affinity.

D. The incorrect nucleotide is removed by the 5’-3’-exonuclease nuclease activity

Choose true and false