A bacterial histidine mutant was plated on minimal medium and a single colony grew. You decide to sequence the histidine biosynthetic gene of the revertant and discover that the original mutation is still present.  This colony must have been able grow due to a

TLS Online TPP Program

#Question id: 13092

#Unit 13. Methods in Biology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

TLS Online TPP Program

#Question id: 12792

#Unit 10. Ecological Principles

Many  homeowners mow  their  lawns  during the  summer and  collect  the  clippings,  which  are  then  hauled to  the local landfill. Which of the following actions would most benefit the local ecosystem?

TLS Online TPP Program

#Question id: 27024

#Unit 10. Ecological Principles

Monophagous herbivores, such herbivore feed on one plant species or just a few closely related species, examples are

TLS Online TPP Program

#Question id: 19900

#Unit 12. Applied Biology

Which one of the following is a sensor?

TLS Online TPP Program

#Question id: 19984

#Unit 2. Cellular Organization

Genome is constant value or remain constant till new evolution break called