#Question id: 4401
#Unit 3. Fundamental Processes
Which of the following occur(s) during Pol II transcription preinitiation complex formation?
a. TFIIA binds to TFIIB. b. TFIIB unwinds the DNA.
c. TFIIB contacts both TATA box–binding factor and DNA.
d. DNA bends.
#Question id: 4402
#Unit 3. Fundamental Processes
Chromatin-mediated repression of transcription involves
a. modification of lysine residues in histones.
b. large, multiprotein complexes.
c. acetylation of histone tails.
d. deacetylation of histone tails.
#Question id: 4403
#Unit 3. Fundamental Processes
Which of the following does (do) not require a DNA helicase activity?
a. SWI/SNF function b. Pol II open-complex formation
c. transcription-factor binding to DNA
d. deacetylation of histone tails
#Question id: 4404
#Unit 3. Fundamental Processes
The yeast two-hybrid system can be used to identify
a. proteins that interact with a known or “bait” protein.
b. proteins that interact with a “fish” domain.
c. cDNAs that encode interacting proteins.
d. co-activators and co-repressors.
#Question id: 4405
#Unit 3. Fundamental Processes
Which of the following can occur when steroid hormone binds to its cognate receptor?
a. conformational changes in the receptor
b. translocation of the receptor out of the cell membrane
c. binding of the receptor to DNA response elements
d. stimulation of transcription
#Question id: 4406
#Unit 3. Fundamental Processes
An electrophoretic mobility shift assay (EMSA) was conducted to check for the binding of TFIIB to a segment of a DNA promoter region. Which of the following approaches could be used to confirm that TFIIB is indeed binding?