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TLS Online TPP Program
#Question id: 18957
#Unit 14. Methods in Biology
A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4x103kb) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are
TLS Online TPP Program
#Question id: 18958
#Unit 14. Methods in Biology
The number of clones to represent this fragment in genomic library with a probability of 95% are
TLS Online TPP Program
#Question id: 18959
#Unit 14. Methods in Biology
Vectors used for construction of genomic libraries, and their capacity is given match them correctly
| A- λ EMBL series | I - 300kb - 1,400 kb |
| B - Cosmids | II - 45 kb |
| C - BACs and YACs (high capacity vectors) | III - 20kb |
TLS Online TPP Program
#Question id: 18960
#Unit 14. Methods in Biology
cDNA library do not require very large vectors hence which of the following is commonly used in cDNA library
TLS Online TPP Program
#Question id: 18961
#Unit 14. Methods in Biology
When a cDNA library is generated by first removing those mRNA or cDNA sequences that are common to two sources, e.g., two different Cell types, such a library is called
TLS Online TPP Program
#Question id: 18962
#Unit 14. Methods in Biology
1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA,
2. Their large size makes the handling procedures during cloning tedious and cumbersome
3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned.
These three problems have been resolved by deleting