TLS Online TPP Program

#Question id: 6976


What is the major concern of using retroviral vectors to deliver or activate pluripotent genes?

#Unit 5. Developmental Biology
  1. Not very efficient and needs repeated transfections
  2. Plasmid diluted as iPS cells divide
  3. Vector and transgenes remain in the genome and can be reactivated in differentiated cells
  4. The vector integrates but is excised by the transposase

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TLS Online TPP Program

#Question id: 2233

#Unit 2. Cellular Organization

Membrane proteins may interact with biomembranes through

a. covalently attached fatty acid molecules.

b. covalently attached lipid molecules.

c. noncovalent protein-protein interactions.

d. hydrophobic α-helical domains.

e. noncovalent protein-lipid interactions.

TLS Online TPP Program

#Question id: 2234

#Unit 2. Cellular Organization

Which of the following statement(s) is (are) true of transmembrane proteins?

a. All contains a hydrophobic α helix.

b. Some serve as membrane attachment sites for peripheral membrane proteins.

c. Some contain more than one membrane-spanning domain.

d. All are asymmetrically oriented in the lipid bilayer.

TLS Online TPP Program

#Question id: 2235

#Unit 2. Cellular Organization

The generation and maintenance of a membrane electric potential requires which of the following:

a. active pumping of ions       

b. ion-specific membrane channel proteins

c. ATP                                    

d. a generally permeable membrane

TLS Online TPP Program

#Question id: 2236

#Unit 2. Cellular Organization

The partition coefficient K, the equilibrium constant for partitioning between oil and water, for butyric acid is about 10–2, and for 1,4-butanediol it is about 10–4.  You add liposomes containing only water to a solution with an initial concentration of 1 mM butyric acid and 100 mM 1,4-butanediol outside the liposomes.  What is the relative rate of diffusion of the two substances into the liposome interior?

TLS Online TPP Program

#Question id: 2237

#Unit 2. Cellular Organization

Glucose enters erythrocytes via a GLUT-1 uniporter. As the levels of glucose in the bloodstream decrease between meals, what happens to the glucose in the cells?

TLS Online TPP Program

#Question id: 2238

#Unit 2. Cellular Organization

Consider the transport of glucose into an erythrocyte by facilitated diffusion.  When the glucose concentrations are 5 mM on the outside and 0.1 mM on the inside, the free-energy change for glucose uptake into the cell is:  (These values may be of use to you:  R = 8.315 J/mol·K; T = 298 K; 9 (Faraday constant) = 96,480 J/V; N = 6.022×10²³ /mol.)