TLS Online TPP Program

#Question id: 15622

#Unit 13. Methods in Biology

9 In your study of a new bacterial species you have identified a transducing phage that you call Px. In addition you have worked out methods to make random transposon insertions into the bacterial genome. You have generated two different transposon insertion collections one with 105 random Tn5 (Kanr) insertions and the other with 105 random Tn10 (Tetr) insertions. You grow Px phage on the mixed collection of Tn5 insertions and use the resulting phage lysate to infect the mixed collection of Tn10 insertions. You select 10,000 Kanr  transductants and find that 80 of them are Tets. Use this information to estimate the total size of the bacterial genome assuming that both Tn5 and Tn10 insert randomly and that the average size of a fragment recombined into the recipient genome during Px transduction is 55 kbp. (Tn5 is about 5 kbp and Tn10 is 10 kbp.)

TLS Online TPP Program

#Question id: 2963

#Unit 2. Cellular Organization

Eukaryotic chromosomes are composed of which of the following macromolecules?

TLS Online TPP Program

#Question id: 3971

#Unit 3. Fundamental Processes

Sigma factors present in bacteria are proteins required to:

TLS Online TPP Program

#Question id: 23223

#Unit 2. Cellular Organization

What will be the surface area/volume ratio of a cocci bacterium with 4 micrometer diameter? 

TLS Online TPP Program

#Question id: 28371

#Unit 5. Developmental Biology

At which cell of the embryo where the Gurken protein is diffuses and do not allow to synthesizes of pipe protein ?