The functions of tropomyosin in skeletal muscle include;

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#Question id: 1951

#Unit 4. Cell Communication and Cell Signaling

A therapeutic approach in rheumatoid arthritis (RA) is to:

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#Question id: 2336

#Unit 2. Cellular Organization

Which of the following exhibit the 9 + 2 structure of microtubules?        

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#Question id: 19186

#Unit 13. Methods in Biology

In another method of quantitative PCR, reporter-quencher set up is used. Which of the statement holds true for this methodology?

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#Question id: 11582

#Unit 7. System Physiology – Animal

Following a stressful stimulus, the fight-or-flight response will cause ________.

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#Question id: 13091

#Unit 13. Methods in Biology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

Which colony’s plasmid do you actually want to use for your studies?