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TLS Online TPP Program

#Question id: 32223

What is the purpose of N-linked Glycosylation?

#Unit 2. Cellular Organization
  1. N-linked oligosaccharide establish a “glyco-code” that marks the progression of protein unfolding.
  2. The presence of oligosaccharides tends to make a glycoprotein more susceptible to digestion by proteolytic enzymes.
  3. N-linked glycosylation making folding intermediates more soluble, thereby promoting their aggregation. 
  4. A small N-linked oligosaccharide protruding from the surface of a glycoprotein can limit the approach of other macromolecules to the protein surface.
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TLS Online TPP Program

#Question id: 13033

#Unit 13. Methods in Biology

The sample is then fractionated by electrophoresis through a _______. As all the proteins now have an _______ to mass ratio, they are separated on the basis of their _____.
TLS Online TPP Program

#Question id: 13034

#Unit 13. Methods in Biology

Which the following statements is/are true about the stacking gel?
i. The stacking gel solution contains acrylamide.
ii. It is on the top of the separating gel.
iii. It is used to dilute the protein sample before it enters the main separating gel.
iv. It is into this gel that the wells are formed and the proteins loaded.
TLS Online TPP Program

#Question id: 13035

#Unit 13. Methods in Biology

Each band on the gel represents a different protein (or protein subunit); _____ proteins move through the gel more _____ than_____ proteins and therefore are found nearer the _____ of the gel.
TLS Online TPP Program

#Question id: 13036

#Unit 13. Methods in Biology

In isoelectric focusing the protein will move until
TLS Online TPP Program

#Question id: 13037

#Unit 13. Methods in Biology

Which of the following proteins will be most far away from the positively charged electrode during isoelectric focusing in a buffer with pH of 5?
TLS Online TPP Program

#Question id: 13038

#Unit 13. Methods in Biology

Please arrange the following steps about ELIZA (Enzyme-linked immunosorbent assay) in chronological order
i. incubate with antibody-enzyme complex that binds primary antibody
ii. coat surface with antigen, block unoccupied sites with nonspecific protein
iii. add substrate, formation of colored product indicates presence of specific antigen
iv. incubate with primary antibody against specific antigen