Coated vesicles that transport material early in the secretory pathway, budding from Golgi compartments, known as:

TLS Online TPP Program

#Question id: 23682

#Unit 13. Methods in Biology

Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to

TLS Online TPP Program

#Question id: 23683

#Unit 13. Methods in Biology

What is the enzymatic function of restriction enzymes?

TLS Online TPP Program

#Question id: 23684

#Unit 13. Methods in Biology

How does a bacterial cell protect its own DNA from restriction enzymes?

TLS Online TPP Program

#Question id: 23685

#Unit 13. Methods in Biology

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?

I. Transform bacteria with recombinant DNA molecule.

II. Cut the plasmid DNA using restriction enzymes.

III. Extract plasmid DNA from bacterial cells.

IV. Hydrogen-bond the plasmid DNA to non plasmid DNA fragments.

V. Use ligase to seal plasmid DNA to non plasmid DNA.

TLS Online TPP Program

#Question id: 23686

#Unit 13. Methods in Biology

Bacteria containing recombinant plasmids are often identified by which process?

TLS Online TPP Program

#Question id: 23687

#Unit 13. Methods in Biology

Bacteria that contain the plasmid, but not the eukaryotic gene, would grow