TLS Online TPP Program

#Question id: 32406


Find the order in which they appear in the TCA cycle.


#Unit 1. Molecules and their Interaction Relevant to Biology
  1. FACBDE
  2. CFABED
  3. CAFBDE
  4. ACFEBD
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TLS Online TPP Program

#Question id: 139

#Unit 1. Molecules and their Interaction Relevant to Biology

 A sequence of amino acids with a relatively high hydropathy is very likely to function by

TLS Online TPP Program

#Question id: 14779

#Unit 13. Methods in Biology

In a certain college, 4% of the men and 1% of the women are taller than 1.8m. Further 60% of the students are woman. If a student is selected at random and is taller than 1.8m, the probability that the student is women is

TLS Online TPP Program

#Question id: 28379

#Unit 5. Developmental Biology

 during drosophila development, Various signalling pathway occur one of them a protein is act as an autocrine fashion and maintaining its own expression, this protein is known as___

TLS Online TPP Program

#Question id: 32372

#Unit 2. Cellular Organization

Endocytosis assembly factors recruit which of the following protein so that the endocytic vesicles invaginate from the membrane?

TLS Online TPP Program

#Question id: 13090

#Unit 13. Methods in Biology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
                              
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
 
What is the Colony 3’s plasmid is;