Match the following

#Question id: 10308

#Unit 1. Molecules and their Interaction Relevant to Biology

Oxidative decarboxylation of pyruvate to acetyl-CoA by the PDH complex, how the pyruvate dehydrogenase complex carries out the five consecutive reactions in the decarboxylation and dehydrogenation of pyruvate?

 A) Pyruvate reacts with the bound thiamine pyrophosphate (TPP) of pyruvate dehydrogenase, undergoing decarboxylation to the hydroxyethyl derivative

 B) A transesterification in which the —SH group of CoA replaces the —SH group of E2 to yield acetyl-CoA and the fully reduced (dithiol) form of the lipoyl group

 C) The reduced FADH2 of E3 transfers a hydride ion to NAD1 , forming NADH, the enzyme complex is now ready for another catalytic cycle

 D) The transfer of two electrons and the acetyl group from TPP to the oxidized form of the lipoyllysyl group of the core enzyme, dihydrolipoyl transacetylase, to form the acetyl thioester of the reduced lipoyl group

 E) Dihydrolipoyl dehydrogenase promotes transfer of two hydrogen atoms from the reduced lipoyl groups of E2 to the FAD prosthetic group of E3 , restoring the oxidized form of the lipoyllysyl group of E2

 Find out the correct sequence of action of this complex enzyme?

#Question id: 804

#Unit 1. Molecules and their Interaction Relevant to Biology

With respect to microRNAs, which, if any, of the following statements, is false?

a) A microRNA normally works by binding to perfectly complementary sequences within an RNA transcript, usually an mRNA.

b) Like the great majority of mRNAs an miRNA is usually produced as a larger precursor RNA that is capped and has a 3’ poly(A) tail.

c) The precursor miRNA undergoes different types of post-transcriptional cleavage by endoribonucleases that are specific for double-stranded target sequences.

d) A nuclear endoribonuclease called dicer cleaves the miRNA precursor so that it forms a stem-loop RNA.

#Question id: 27530

#Unit 1. Molecules and their Interaction Relevant to Biology

Match the Group 1 with appropriate solution of Group 2

Group 1                                                  Group 2

(1) Agar                                               (P)  Plant cell wall

(2) Raffinose                                       (Q)  Trisaccharides 

(3) Cellulose                                       (R)  Xanthomonas Compestris

(4) Xanthum Gum                             (S) seaweeds 

#Question id: 30115

#Unit 1. Molecules and their Interaction Relevant to Biology

The antibacterial trimethoprim is an inhibitor of 

#Question id: 10306

#Unit 1. Molecules and their Interaction Relevant to Biology

pyruvate dehydrogenase (PDH) complex requires five different coenzymes or prosthetic groups and these enzymesalso require four different vitamins in human nutrition are vital components of this system:

#Question id: 4109

#Unit 1. Molecules and their Interaction Relevant to Biology

When E. coli is grown on a medium containing a mixture of glucose and lactose, it proliferates with complex kinetics. The bacteria proliferate faster at the beginning than at the end, and there is a lag between these two phases when they virtually stop dividing. Assays of the concentrations of the two sugars in the medium show that glucose falls to very low levels after a few cell doublings, but lactose remains high until near the end of the experimental time course (not shown). Although the concentration of lactose is high throughout most of the experiment, β-galactosidase, which is regulated as part of the Lac operon, is not induced until more than 100 minutes have passed. Choose correct explanation why the Lac operon is not induced by lactose during the rapid initial phase of bacterial proliferation

A. The rapid bacterial growth at the beginning of the experiment results from the metabolism of glucose. The slower growth at the end results from metabolism of lactose.

B. CAP and the Lac repressor mediate induction in the situation

C. The bacteria stopped growing in the middle of the experiment because they ran out of glucose but did not yet possess the enzymes necessary for lactose metabolism

D. Induction of the Lac operon requires that two conditions be met: lactose must be present and glucose must be absent.