In budding yeast, microtubules are nucleated at an MTOC that is embedded in the nuclear envelope as a small, multilayered structure called:

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#Question id: 19845

#Unit 13. Methods in Biology

SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.

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#Question id: 19846

#Unit 13. Methods in Biology

Variants of PCR has been developed on the basis of___

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#Question id: 19865

#Unit 13. Methods in Biology

Match the following activity of variants in Column I with their variants of PCR in Column II given below;

COLUMN I	COLUMN II
A) Enhance efficiency	i) Multiplex PCR
B) Sequence specific amplification	ii) Hot-start PCR
C) Mutational analysis	iii) Megaprimer PCR
	iv) qPCR with SYBR
	v) AS-PCR
	vi) RLM-RACE

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#Question id: 19866

#Unit 13. Methods in Biology

Which of the following variants of PCR has been devised to increase the specificity of PCR without lowering the efficiency by variation annealing temperature?

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#Question id: 19867

#Unit 13. Methods in Biology

Which of the following variants of PCR were devised to prevent non-specific amplification at low temperature?

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#Question id: 19868

#Unit 13. Methods in Biology

Which one of the following statements about FRET is correct?

a) FRET is possible only when the distance between donor and acceptor are within 1 to 10 A0

b) For FRET, GFP chromophore contain tripeptide sequence is -Gly-Phe-Pro-

c) qPCR based on FRET

d) Efficiency of FRET is directly proportional to the sixth power of the distance between donor and acceptor.