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TLS Online TPP Program
#Question id: 18961
#Unit 13. Methods in Biology
When a cDNA library is generated by first removing those mRNA or cDNA sequences that are common to two sources, e.g., two different Cell types, such a library is called
TLS Online TPP Program
#Question id: 18962
#Unit 13. Methods in Biology
1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA,
2. Their large size makes the handling procedures during cloning tedious and cumbersome
3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned.
These three problems have been resolved by deleting
TLS Online TPP Program
#Question id: 18963
#Unit 13. Methods in Biology
The use of wild type pTi as a vector presents the following three problems.
1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA, which causes a disorganised growth and a loss of regeneration potentiaLof the cells having T-DNA in their genomes.
2. Their large size makes the handling procedures during cloning tedious and cumbersome.
3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned
In the above mention’s problems, 1 and 3 can removed by disarming and creating MCS, to overcome from 2nd problem need to develop
TLS Online TPP Program
#Question id: 18964
#Unit 13. Methods in Biology
Size of agrobacterium can be reduced by binary vector formation; these binary vectors can be formed by phenomenon of
TLS Online TPP Program
#Question id: 18969
#Unit 8. Inheritance Biology
Consider the ABO “blood type” gene. This single gene has three alleles called A, B, and O. There are four resultant “blood types” (phenotypic classes), as follows:C
Assume Hardy-Weinberg equilibrium. In Norway, the frequencies of the A and B alleles are 0.26 and 0.07, respectively. What is the frequencies of these six genotypes (AA, AO, BB, BO, AB, and OO);
TLS Online TPP Program
#Question id: 18970
#Unit 8. Inheritance Biology
Consider the ABO “blood type” gene. This single gene has three alleles called A, B, and O. There are four resultant “blood types” (phenotypic classes), as follows:
Assume Hardy-Weinberg equilibrium, the frequencies of the A and B alleles are 0.26 and 0.07, respectively. What is the frequencies of the four blood types such as A, B, AB and O in Norway;