Find correct True and False statements

A. Human females have 23 different chromosomes, whereas human males have 24.

B. In the living cell, chromatin usually adopts the extended “beads-on-astring” form.

C. The four core histones are relatively small proteins with a very high proportion of positively charged amino acids; the positive charge helps the histones bind tightly to DNA, regardless of its nucleotide sequence.

D. Nucleosomes bind DNA so tightly that they cannot move from the positions where they are first assembled.

TLS Online TPP Program

#Question id: 34173

#Unit 3. Fundamental Processes

The 3’–5’ exonucleolytic activity is found in which subunit of bacterial DNA Polymerase III

TLS Online TPP Program

#Question id: 34174

#Unit 3. Fundamental Processes

Once bound to a primer:template junction, DNA polymerase interacts tightly with much of the double stranded portion of the DNA by

TLS Online TPP Program

#Question id: 34175

#Unit 3. Fundamental Processes

A. For processive DNA synthesis, proofreading occurs without releasing the DNA from the polymerase

B. When a mismatched base pair is present in the polymerase active site, the primer:template junction is destabilize

C. The DNA polymerase active site binds such a mismatched template poorly, but the exonuclease active site has a 10-fold higher affinity.

D. The incorrect nucleotide is removed by the 5’-3’-exonuclease nuclease activity

Choose true and false

TLS Online TPP Program

#Question id: 34176

#Unit 3. Fundamental Processes

A. The lagging strand must be synthesized in the form of Okazaki fragments. 

B. Both strands are synthesized discontinuously. 

C. Not all of the fragment population represents bona fide Okazaki fragments; some are pseudofragments

D. Primers start with the sequence pppAG positioned opposite the sequence 3’–GTC-5’ in the template

Choose true and false

TLS Online TPP Program

#Question id: 34177

#Unit 3. Fundamental Processes

Which of the following combination of the statements regarding polA mutants is correct

A. fail to join their Okazaki fragments 

B. fail to removes the RNA primer

C. fail to removes the mismatch errors

D. fail to synthesis Okazaki fragments 

TLS Online TPP Program

#Question id: 34178

#Unit 3. Fundamental Processes

A. SSBs interact with ssDNA in a sequence-independent manner. 

B. SSBs primarily contact ssDNA through electrostatic interactions with the phosphate backbone and stacking interactions with the DNA bases.

C. SSBs interact with ssDNA in a sequence dependent manner via hydrogen bonds with phosphate backbone

D. SSBs shows sigmoidal behaviour of binding

Choose true and false