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#Question id: 16138
#Unit 13. Methods in Biology
You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
Would you expect STS51 and STS52 to be present in BAC D?
TLS Online TPP Program
#Question id: 16139
#Unit 13. Methods in Biology
Many mouse genes are “tissue-specific,” that is, they are present throughout the body but are expressed in only one of the animal’s many tissue types. (Other mouse genes are expressed throughout the body, or in multiple tissues.) Geneticists can study the regulation of a mouse gene by fusing the gene’s promoter region to the LacZ coding sequence and injecting the construct to create a transgenic mouse. Fusion of the mouse amylase promoter to LacZ yielded a Pamylase-LacZ construct.
Mice heterozygous for the resulting Pamylase-LacZ transgene displayed the LacZ expression exclusively in the pancreas. Would you expect homozygotes for the transgene to also display LacZ expression in the pancreas?.
TLS Online TPP Program
#Question id: 16140
#Unit 13. Methods in Biology
Many mouse genes are “tissue-specific,” that is, they are present throughout the body but are expressed in only one of the animal’s many tissue types. (Other mouse genes are expressed throughout the body, or in multiple tissues.) Geneticists can study the regulation of a mouse gene by fusing the gene’s promoter region to the LacZ coding sequence and injecting the construct to create a transgenic mouse. Fusion of the mouse amylase promoter to LacZ yielded a Pamylase-LacZ construct.
You are surprised to observe that mice homozygous for the transgene insertion display a serious heart defect. (Heterozygotes have normal hearts.) which one is a possible explanation?
a) LacZ overexpression (with two copies of the transgene) causes the defect.
b) the insertion disrupted a gene that is haploinsufficient.
c) the insertion disrupted a gene that is haplosufficient.
d) LacZ expression inhibition (with two copies of the transgene) causes the defect.
Which of the following statements is correct predict about the heart defect?
TLS Online TPP Program
#Question id: 17270
#Unit 13. Methods in Biology
which of the following are the three important components of Electrophoresis
TLS Online TPP Program
#Question id: 17271
#Unit 13. Methods in Biology
Electrophoretic resolution can be mainly change by changing the
TLS Online TPP Program
#Question id: 17272
#Unit 13. Methods in Biology
We need to minimum buffering capacity is required so that the pH value of the samples analyzed does not have any influence on the system,
What would happen if we used the higher buffering concentration
a) more electric power is applied, resulting in higher heat development.
b) less electric power for less time is applied, resulting in very high heat development
c) higher buffering concentration need more electric power resulting increases in the temperature will increase the mobility and rate of migration of ions.
d) using the higher buffering concentration shows no affect at all