TLS Online TPP Program
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TLS Online TPP Program
#Question id: 41087
#Unit 12. Biochemical Engineering
Q. Consider the following statements regarding the principles and applications of industrial fermentation technologies for enzyme production:
P. Submerged Fermentation (SmF) is currently the primary commercial method for cultivating both bacteria and filamentous fungi, relying on large stirred-tank bioreactors that can exceed 100 m³ in volume.
Q. In Solid-State Fermentation (SSF), the substrates and nutrients must be completely dissolved in a free-flowing liquid broth to closely mimic the natural ecological niche of filamentous fungi.
R. Industrial enzymes produced via these fermentation methods function as biological catalysts by operating under high temperature and extreme pressure conditions to maximize the rate of chemical reactions.
S. SSF processes frequently utilize moist, solid agro-residues such as wheat bran or molasses, operating with low free water availability which is particularly common for the production of fungal enzymes.
Which of the above statements are strictly correct?
TLS Online TPP Program
#Question id: 41088
#Unit 12. Biochemical Engineering
Q. Which of the following microorganisms is primarily utilized for the 11alpha-hydroxylation of progesterone, representing the core step in the commercial manufacturing of cortisone?
TLS Online TPP Program
#Question id: 41089
#Unit 12. Biochemical Engineering
Q. Which microorganism is responsible for introducing a hydroxyl group at the C16 position during the synthesis of triamcinolone intermediates?
TLS Online TPP Program
#Question id: 31766
#Unit 12. Biochemical Engineering
Sugarcane molasses containing 50% sucrose, 1 % invert sugars, 18% water and 31 % other solids is mixed with corn steep liquor containing 2.5% invert sugars, 50% water and 47.5% other solids to produce a diluted sugar mixture containing 2% invert sugars. 125 kg corn steep liquor and 45 kg molasses are fed into the mixing tank. How much water should be added to the mixing tank to produce the desired diluted sugar mixture?
TLS Online TPP Program
#Question id: 31767
#Unit 12. Biochemical Engineering
A bacteria l culture, with the molecular formula - C4.4 H7.3 01.2 N0.86 is cultivated under aerobic conditions with hexadecane (C16H34) as substrate. The growth can be described by equation: C16H34 + 16.28O2 + 1.42NH3 ➔ 1.65C4.4 H7.3O1.2 N0.86 + 8.74CO2 + 13.11 H2O. Assuming 100% conversion, the yield of cell mass from hexadecane will be
TLS Online TPP Program
#Question id: 31773
#Unit 12. Biochemical Engineering
A strain of E coli is cultured in a 15 m3 mechanically stirred bioreactor. Under the operating conditions, the value of kLa is 0.17 s-1. Oxygen solubility in the broth is 8x 10-3 kgm -3 . If the specific rate of O2 uptake is 12.5 mmoles.g-1 .h-1, what is the maximum possible cell concentration?
