A. The lagging strand must be synthesized in the form of Okazaki fragments. 

B. Both strands are synthesized discontinuously. 

C. Not all of the fragment population represents bona fide Okazaki fragments; some are pseudofragments

D. Primers start with the sequence pppAG positioned opposite the sequence 3’–GTC-5’ in the template

Choose true and false

TLS Online TPP Program

#Question id: 18954

#Unit 13. Methods in Biology

The above diagram showing which of the following technique

TLS Online TPP Program

#Question id: 18955

#Unit 13. Methods in Biology

(i) (RFLPs), (ii) (RAPDs), (iii) (VNTR) DNA, and (iv) (SSRs) are the examples of

TLS Online TPP Program

#Question id: 18956

#Unit 13. Methods in Biology

It is possible to calculate the number of clones that must be present in a gene library to give a probability of obtaining a particular DNA sequence. This formula is:

 

where N is the number of recombinants, P is the probability and f is the fraction of the genome in one insert. Thus for the E. coli DNA chromosome of 5 × 10 ⁶ bp andan insert size of 20 kb ( f = 0.004), the number of clones needed ( N ) would be 1 × 10 ³ ,with a probability of P will be

TLS Online TPP Program

#Question id: 18957

#Unit 13. Methods in Biology

A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4x103kb) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are

TLS Online TPP Program

#Question id: 18958

#Unit 13. Methods in Biology

The number of clones to represent this fragment in genomic library with a probability of 95% are

TLS Online TPP Program

#Question id: 18959

#Unit 13. Methods in Biology

 Vectors used for construction of genomic libraries, and their capacity is given match them correctly

A- λ EMBL series                                                                                   I - 300kb - 1,400 kb

B - Cosmids                                                                                                 II - 45 kb

C – BACs and YACs (high capacity vectors)                                             III - 20kb