#Question id: 13076
#Unit 13. Methods in Biology
Restriction fragment length polymorphism denotes that a
single restriction enzyme produces fragments of different lengths from the same
stretch of genomic DNA of different strains of a species or from different
related species. RFLPs are detected as follows:
i. Large
molecular weight genomic DNA is isolated from several strains or related
species;
ii. The
fragments in these digests are separated through electrophoresis
iii. These
'DNAs are then digested with a selected restriction enzyme
iv. Exposed
to a suitably radio-labelled appropriate DNA probe under conditions favouring
DNA: DNA hybridization
v. The
resulting gel lanes are transferred and fixed to a suitable solid support and
vi. The free
probes are removed
vii. The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.
#Question id: 18945
#Unit 13. Methods in Biology
#Question id: 28349
#Unit 5. Developmental Biology
After the organization of microtubules, the two mRNA bicoid and oskar complexed with microtubules motor protein in oocyte, which localizes these mRNA, select the correct prediction about oskar mRNA?
#Question id: 1590
#Unit 4. Cell Communication and Cell Signaling
An epitope associates with which part of an antibody?
#Question id: 29049
#Unit 10. Ecological Principles