Kragl and colleagues (2009)
performed an experiment in which they transplanted limb tissue from a
salamander whose cells expressed green fluorescent protein (GFP) into different
regions of limbs of normal salamanders that did not have the GFP transgene. If
they transplanted the GFP-expressing limb cartilage into a salamander limb that
did not contain the GFP transgene, the GFP-expressing cartilage would integrate
normally into the limb skeleton. They later amputated the limb through the
region containing GFP-marked cartilage cells. The blastema was found to contain
GFP-expressing cells, and when the blastema differentiated, the only
GFP-expressing cells found were in the limb cartilage. Similarly, GFP-marked
muscle cells gave rise only to muscle, and GFP-marked epidermal cells only
produced the epidermis of the regenerated limb. Which of the following
phenomenon is proved according to experiment?
#Unit 5. Developmental Biology
These results suggest that nAG alone is the primary mitogen responsible for nerve-dependent regeneration.
The blastema is not a collection of unspecified multipotent progenitor cells. Rather, the cells retain their specification, and the blastema is a heterogeneous assortment of restricted progenitor cells.
The blastema is a collection of unspecified multipotent progenitor cells. Rather, the cells lost their specification, and the blastema is a homogeneous assortment of restricted progenitor cells.
Salamanders accomplish epimorphic regeneration by cell dedifferentiation to form a regeneration blastema, which in this case is an aggregation of relatively undifferentiated cells derived from the originally differentiated tissue that then proliferates and redifferentiates into the new limb parts.
Answer:- Option(s): 2
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