An enzyme-catalyzed reaction was carried out with the substrate concentration initially a thousand times greater than the Km for that substrate.  After 9 minutes, 1% of the substrate had been converted to product, and the amount of product formed in the reaction mixture was 12 micromol.  If, in a separate experiment, one-third as much enzyme and twice as much substrate had been combined, how long would it take for the same amount (12 micromol) of product to be formed?

TLS Online TPP Program

#Question id: 20823

#Unit 12. Biochemical Engineering

In a plug flow bioreactor running at steady state, 12.5% cells are recycled back to the inlet. If the cells grow at μmax=1 h-1 throughout the length of the reactor (L=124 cm), then the flow velocity should be

TLS Online TPP Program

#Question id: 20824

#Unit 12. Biochemical Engineering

In a CSTR, first order reaction takes place converting A to B. If at a dilution rate (D)= 0.5 h-1, 50% of A gets converted to B, then the rate constant ‘k’ of the reaction is:

TLS Online TPP Program

#Question id: 20827

#Unit 12. Biochemical Engineering

A new spherical resin (of radius 300 urn) has been developed in which only the outer layer is activated to a depth of 100 um (as shown in figure). The fraction of the activated volume is: 

TLS Online TPP Program

#Question id: 20828

#Unit 12. Biochemical Engineering

In a fed batch cultivation, a specific growth rate of 0.2/h needs to be maintained. At the start of fedbatch cultivation, 200 mL/h of media is fed to 1000 mL of working volume. Quasi steady state shall be obtained if:

TLS Online TPP Program

#Question id: 20836

#Unit 12. Biochemical Engineering

Two columns of 1 m and 2 m height and of equal diameter are packed with beads containing immobilized enzyme. Substrate was fed to these two columns at flow rates of 10 mL/min (1 m column) and 20 mL /min (2 m column) and the corresponding conversion efficiencies obtained were 30% and 40% respectively. This demonstrates that the reaction is:

TLS Online TPP Program

#Question id: 20562

#Unit 12. Biochemical Engineering

Scale up of a fermenter is done based on constant impeller tip speed. If the diameter of the impeller is increased by 10 fold, the agitator speed will: