TLS Online TPP Program

#Question id: 40548


Molecular beacons (MB) and Taqman (TQ) are used as probes in Real time PCR experiments. Both these probes are based on the principle of FRET and employ a fluorophor (F) and a quencher (Q). However the mechanisms by which they function are different as illustrated below

At what stage of the PCR we would be able to detect fluorescence?

#Unit 13. Methods in Biology
  1. Annealing step for both.
  2. Extension step for both.
  3. Annealing for A and Extension for B.
  4. Extension for A and Annealing for B.