TLS Online TPP Program

#Question id: 21396


Two immobilized enzyme columns with equal enzyme loading and same column volume are run at the same feed rate and same inlet substrate concentration. It is observed that the taller and thinner column gives better conversion. This demonstrates that

#Unit 12. Biochemical Engineering
  1. Column packing efficiency is not good,
  2. Enzyme deactivation is taking place
  3. Immobilized enzyme has external diffusion which reduces the enzymatic conversion
  4. Immobilized enzyme has internal pore diffusion which reduces the enzymatic conversion rate,