Aqueous Two Phase System (ATPS) is used to isolate a protease from a fermentation broth. The partition coefficient (K) is 2.5. For 80 % recovery of protease in a single step, the volume ratios of upper and the lower phases should be:

TLS Online TPP Program

#Question id: 29437

#Unit 6. System Physiology – Plant

Important chemical reactions involved in Nitrate assimilation at roots are given

a. a & b both is Nitrate reductase 

b. a & b both is NADPH nitrite reductase 

c. a is Nitrate reductase and reaction takes place in plastid 

d. a is Nitrate reductase and b is NADPH nitrite reductase

Choose the correct statement made regarding the above reactions of the following

TLS Online TPP Program

#Question id: 29438

#Unit 6. System Physiology – Plant

Symbiotic nitrogen fixation in legume nodules involves Root hair cellular responses to bacterial Nod factor and this response is time-based phenomenon

Response	Timing
I. Membrane potential depolarization	a. Observed in root hairs within 1 minute of NF application
II. Ca2+ spiking in and around root hair cell nucleus	b. Begins within ~10 minutes after NF exposure
III. Root hair growth perturbation	c. Occur over a period of hours
IV. Reduction in rate of reactive oxygen production	d. Observed in root hairs within 1 minute of NF application

Match the Response with their appropriate timing

TLS Online TPP Program

#Question id: 29439

#Unit 6. System Physiology – Plant

Nitrogenase, a complex metal containing enzyme is involved in conversion of N2 to NH3•

a. Nitrogenase is composed of two component proteins

b. Nitrogenase is a complex consisting of the MoFe protein (dinitrogenase or component I) a heterotetramer

c. Nitrogenase is a complex consisting of the MoFe protein (dinitrogenase or component I) a heterotetramer

d. Fe protein (dinitrogenase reductase or component II) a heterodimer

Which of the combination of above statement about Nitrogenase is INCORRECT

TLS Online TPP Program

#Question id: 29440

#Unit 6. System Physiology – Plant

Nitrogenase is a complex consisting of the 

(a) MoFe protein (dinitrogenase or component I) a heterotetramer

(b) the Fe protein (dinitrogenase reductase or component II) a heterotetramer

(c) MoFe protein (dinitrogenase or component I) a homodimer.

(d) the Fe protein (dinitrogenase reductase or component II) a homodimer

Which of the following is correct 

TLS Online TPP Program

#Question id: 29441

#Unit 6. System Physiology – Plant

Nitrogenase is a complex consisting of the MoFe protein Fe protein,

Here few of the statement are given regarding Fe protein is given 

(a) contains 2 identical subunits (dimer) – nif H

(b) Each subunit has MW of 30 ~ 72 kD.

(c) Each subunit contains an Fe-S cluster (4Fe and 4S2-).

(d) Each subunit has 2 Mo-Fe-S clusters

Which of the following is true regarding Fe protein

TLS Online TPP Program

#Question id: 29442

#Unit 6. System Physiology – Plant

Some of the following statement are given regarding MoFe protein

(a) 2 pairs of identical subunits (tetramer, nif D & nif K)

(b) A total of MW 180 ~ 235kD

(c) Each subunit has 2 Mo-Fe-S clusters.

(d) MoFe proteins are insensitive to O₂

Which of the following is incorrect