#Question id: 4445
#Unit 3. Fundamental Processes
The active site, which is made up of RPB1 and RPB2, from both these subunits, is found at the base of the pincers within a region called the “active center cleft” . The active site works according to the two-metal ion catalytic mechanism for nucleotide addition proposed for all types of polymerase;
#Question id: 4446
#Unit 3. Fundamental Processes
Transcription start site is designated with the;
#Question id: 4447
#Unit 3. Fundamental Processes
Sequences in the direction in which transcription proceeds are referred to as __?from the start site, and allotted a? value.
#Question id: 4448
#Unit 3. Fundamental Processes
Three defined steps in a transcription cycle; choose correct one
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1a. Closed complex |
In this form, the DNA remains double-stranded, and the enzyme is bound to one face of the helix. I |
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1b. Open complex |
In which the DNA strands separate over a distance of 13 bp around the start site to form the transcription bubble. In the next stage of initiation, |
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2. Promoter escape |
Incorporation of the first 10 or so ribonucleotides is a rather inefficient process, and at that stage, the enzyme often releases short transcripts and then begins synthesis again |
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3. Initial transcribing complex. |
Once an enzyme makes a transcript longer than 10 nucleotides, it is said to have |
#Question id: 4449
#Unit 3. Fundamental Processes
Promoters recognized by polymerase containing s70 share the following characteristic structure;
#Question id: 4450
#Unit 3. Fundamental Processes
According to the correlation between promoter strength and sequence, in all these promoters which one is seems like very weak promoter or non-existing in any organism