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TLS Online TPP Program

#Question id: 20941

IPTG is used as an inducer in the T7 expression system for recombinant protein expression in E.coli. This is because of:

#Unit 13. Advances in Biotechnology
  1. availability of special E.coli cells which have the T7 RNA polymerase gene integrated into their genome under a regulatable promoter
  2. T7 promoter is recognized by E.coli RNA polymerase
  3. High copy number of plasmid allows sequestration of E.coli RNA polymerase by T7 promoter
  4. IPTG facilitates binding of E.coli RNA polymerase to the T7 promoter.
More Questions
TLS Online TPP Program

#Question id: 28636

#Unit 2. Cellular Organization

At which phase of Cdh1 is phosphorylated, Cdh1 dissociates from APC/C, and APC/C becomes inactive?
TLS Online TPP Program

#Question id: 28637

#Unit 2. Cellular Organization

At which phase of Cdh1 is de-phosphorylated and APC/Ccdh1 is active?
TLS Online TPP Program

#Question id: 28638

#Unit 2. Cellular Organization

S phase cyclin-CDKs are inactivated by binding of given which CKIs inhibitor?
TLS Online TPP Program

#Question id: 28639

#Unit 2. Cellular Organization

Degradation of Sic1 is induced by___
TLS Online TPP Program

#Question id: 28640

#Unit 2. Cellular Organization

Which of the following kinase activate CDKs in all organism?
TLS Online TPP Program

#Question id: 28641

#Unit 2. Cellular Organization

Match the following column I and II

 Column I Column II
 I- APC/CCdc20 (a) promotes the metaphase–anaphase transition
 II- APC/CCdh1 (b) responsible for destroying proteins, such as securin
 (c) responsible for ubiquitinating proteins, such as mitotic cyclins 
 (d) Promotes exit from mitosis