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TLS Online TPP Program

#Question id: 9615

In angiosperm plants, flower morphology can be very intricate. If a tree, such as a New Mexico locust, has flowers that share many morphological intricacies with flowers of the sweet pea vine, then the most likely explanation for these floral similarities is the same general explanation for the similarities between the

#Unit 9. Evolution & Diversity of Life Forms
  1. dorsal fins of sharks and of dolphins.
  2. reduced eyes of Australian moles and North American moles.
  3. scales on moth wings and the scales of fish skin.
  4. cranial bones of humans and those of chimpanzees.
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TLS Online TPP Program

#Question id: 12909

#Unit 14. Methods in Biology

According to the diagram, arrange the steps in correct order: 


1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 
2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 
3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).
 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.
TLS Online TPP Program

#Question id: 12910

#Unit 14. Methods in Biology

Match the following:

A. RNA analysisi. Sexing or cell mutation sitesa. Expressed Sequence tag
B. Forensic scienceii. mRNA analysisb. Analysis of DNA from blood
C. Gene discoveryiii. DNA profilingc. Active latent viral infections
D. Single-cell analysisiv. RT–PCRd. Sex determination of unborn
TLS Online TPP Program

#Question id: 12911

#Unit 14. Methods in Biology

Match the following:

A. DNA polymerasesi. Alkaline phosphatasea. RNA-dependent DNA polymerase, used in cDNA synthesis           
B. Reverse transcriptaseii. T4 DNA ligaseb. Thermostable DNA polymerase used in PCR
C. Ligasesiii. AMV-RTc. Removes 5'-phosphates from DNA and RNA
D. Phosphatasesiv. Taq DNA pold. Links 5'-phosphate and 3'-hydroxyl ends via phosphodiester bond
TLS Online TPP Program

#Question id: 12912

#Unit 14. Methods in Biology

Name the enzyme which separates the double-helical DNA into single strands.
TLS Online TPP Program

#Question id: 12913

#Unit 14. Methods in Biology

________  involves the size analysis of restriction fragments produced by several restriction enzymes individually and in combination. 
TLS Online TPP Program

#Question id: 12914

#Unit 14. Methods in Biology

 In Electrophoresis of DNA the gel is first soaked in the alkali then it is transferred to the membrane so that the ________